Frequent gain- and loss-of-function mutations of the BjMYB113 gene accounted for leaf color variation in Brassica juncea

Abstract Background Mustard ( Brassica juncea ) is an important economic vegetable, and some cultivars have purple leaves accumulate more anthocyanins than the green. The genetic evolution of trait in mustard has not been well studied. Result In this study, free-hand sections metabolomics showed that accumulated green ones. gene controlling mustard, Purple Leaves MPL ), was genetically mapped a MYB113-like homolog identified as candidate gene. We three alleles gene, BjMYB113a from cultivar, BjMYB113b BjMYB113c cultivars. A total 45 single nucleotide polymorphisms (SNPs) 8 InDels were found between promoter sequences allele . On other hand, only sequence variation insertion 1,033-bp fragment 3’region Transgenic assay activity studies polymorphism region responsible for up-regulation high accumulation anthocyanin cultivar. increased expression genes biosynthesis pathway including BjCHS , BjF3H BjF3’H BjDFR BjANS BjUGFT consequently led to anthocyanin. However, BjMYB113 compromised by Conclusions Our results contribute better understanding genetics provide useful information further breeding programs mustard.